Thin-layer chromatography of urinary imidazoles.

DURING A STUDY on the metabolism of histidine in a child affected with histidinemia (Beauvais, P., and Humbel, R., to be published), we became interested in the chromatography of imidazoles. In this disease the urine contains numerous imidazoles: histidine and its metabolites, imidazole-pyruvic, imidazole-lactic and imidazole-acetic acids and N-acetylhistidine.’I developed a chromatographic method on thin-layer cellulosewhich permits rapid resolution of these urinary imidazoles.